Troubleshooting
- If scaffold flotation is observed during seeding or early culture, consider the following approaches to promote stability:
* Pre-wet the scaffolds with complete culture media for an extended period prior to cell seeding to enhance saturation and reduce buoyancy.
* Use sterile weighting devices, such as stainless steel or Teflon rings, to gently anchor the scaffolds without compromising sterility or cell viability.
Ensure that any materials used for weighting are biocompatible and have been sterilized according to your laboratory’s protocols. - To promote uniform cell distribution across the scaffold, minimize movement of the culture vessel during the first few hours post-seeding. Furthermore, avoid adding excess culture media too early, as this may dislodge cells from the scaffold surface, particularly at the edges. Smaller media volumes can be used by preparing a more concentrated cell suspension (1-10 × 10⁶ cells/mL) to ensure a droplet based seeding method.
- To promote uniform cell distribution across the scaffold, minimize movement of the culture vessel during the first few hours post-seeding. Furthermore, avoid adding excess culture media too early, as this may dislodge cells from the scaffold surface, particularly at the edges. Smaller media volumes can be used by preparing a more concentrated cell suspension (1-10 × 10⁶ cells/mL) to ensure a droplet based seeding method.
