Thank you for joining us on this collaborative journey. Below are guidelines on how to use our scaffolds in our sample kits. These are not meant to be used in every application, but rather as a launching point for your research with VivoTex Scaffolds. Please reach out to us if you have further questions.

You can also download a printable version of our guide and protocol here.

Intended Use

VivoTexTM scaffolds are custom-designed to augment your research, enhancing results that advance your scientific discoveries. VivoTexTM scaffolds are shipped sterile, placed inside low-attachment tissue culture plates, and can be used directly for cell culture applications. The scaffold kit does not contain any living cells or materials. VivoTexTM scaffolds are suited for research use only. Not approved for applications to humans or animals, or for diagnostic use.

Required Equipment and Materials (not supplied)

• Cell suspension: Prepare cells in suspension at a recommended concentration of 1 × 10⁶ cells/mL in culture medium.
• Culture Medium: It is recommended to use cell culture medium supplemented with 10% (v/v) serum (e.g. Fetal Bovine Serum) and 1% (v/v) antimicrobial agents (e.g., Penicillin-Streptomycin).
• Water bath or heat block
• Sterile Pipettes and pipette tips
• Biosafety cabinet
• Incubator (37°C, 5% CO₂)
• Ethanol for work area sterilization
• Sterile forceps (optional for scaffold handling)
• Sterile 1.5 mL microcentrifuge tubes (optional for scaffold handling)

Required Equipment and Materials (not supplied)

• All steps should be performed using biological aseptic techniques using a Biological Safety cabinet to maintain sterility of the product.
• All kit components can be used at room temperature (18-25°C) Use disposable, individually wrapped, sterile plasticware and sterile tubes, pipettes tips, and forceps.
• The biological performance is dependent on the cells used. It is recommended to always include a 2D culture control (without VivoTexTM scaffolds) to confirm cell attachment capacity using cell-culture treated well plates.
• Wear appropriate Personal Protective Equipment in accordance with the biological hazards introduced in your culture protocol (cell type and culture medium).