Sterilization

• One day before starting your experiment, immerse VivoTex MEW scaffolds scaffolds in 70% ethanol for 30 minutes under sterile conditions.

• Wash the scaffolds with Phosphate Buffer Solution three times (5min per wash) under sterile conditions.

Pre-treatment 1: Etching

• Treat the VivoTexTM scaffolds with 1M NaOH (sterile) for 15-30 minutes under sterile conditions at room temperature.

• Wash the scaffolds with PBS three times (5 min per wash) under sterile conditions.

• Remove PBS from the scaffolds and allow the scaffolds to air dry inside the biosafety cabinet, leaving the lid off the well plate (1-2h).  

• Proceed with the steps for protein adsorption or cell seeding, as outlined below.

Pre-treatment 2: Protein adsorption

• Before starting your experiment, treat the VivoTexTM scaffolds with serum containing media (e.g. 10% FBS) for 2-24 hours under sterile conditions.

• Remove the culture media and proceed with the steps for seeding cells outlined below.